Bluetongue Virus Kit - virotype BTV pan/8 2.0 RT-PCR Kit (100 reactions)

Bluetongue Virus Kit - virotype BTV pan/8 2.0 RT-PCR Kit (100 reactions)

For detection of Bluetongue Virus RNA plus separate identification of the European serotype BTV-8 - options for BTV-3 and BTV-4 are available.

The virotype BTV pan/8 2.0 RT-PCR Kit (FLI-C 114) from INDICAL enables rapid and reliable detection of Bluetongue Virus (BTV) RNA, and differentiation of European serotype BTV-8 specifically, in samples from cattle, sheep and goats.

Save precious lab time with virotype BTV pan/8 2.0 RT-PCR Kit

• Enhanced, ultra-fast protocol with a total RT-qPCR runtime of ~1 hour*
• Can be performed in one cycler run with other virotype 2.0 assays
• Easy-to-use single reaction mix with endogenous internal extraction and amplification control
• Ready-to-use positive and negative controls 

Excellent performance 

• Triplex RT-qPCR for Identification of all known 36 BTV serotypes plus separate detection of BTV-8 in one single PCR run
• Highly sensitive detection of as few as 5 copies per sample
• Usable on most common PCR thermocyclers
• High efficiency on all instruments tested

Flexible with multiple validated sample types 

• Ruminant whole blood (preferred with anticoagulants, e.g., EDTA-blood)
• Tissue samples (spleen, lymph nodes) from cattle, sheep and goats

Due to the high sensitivity of the test, pools of up to 5 individual blood samples may be analyzed.
However, the optimum pool size depends on the regional prevalence for BTV.**

Bluetongue disease and Bluetongue Virus (BTV)

Bluetongue is an infectious, non-contagious disease of ruminants. The agent is the Bluetongue Virus (BTV), a double-stranded RNA virus of the genus Orbivirus of the family Reoviridae which includes 36 known serotypes including atypical BTV. BTV is widely distributed around the world. Sheep, cattle and goats are mainly affected by the disease. Clear clinical signs are usually seen only in sheep. In severe cases the tongue may show intense hyperemia and become cyanotic (Bluetongue).

BTV serotype 8 (BTV-8) is of epidemiological importance in Central Europe and a cause of recent major Bluetongue Disease outbreaks. The virus is transmitted by certain midges of the genus Culicoides. Furthermore, the virus can be spread by contaminated needles and surgery equipment.

*Depending on PCR-Cycler. See Handbook for detailed information. 

**Pool recommendations and regulations may vary between countries.