Air Sampling- Increasing Field Sampling Options
GeneWorks collaborators at La Trobe University have recently published a research note on an air sampling method, by which viral particles were collected using a liquid cyclonic impinging device. This method was successfully used to detect Infectious laryngotracheitis (ILT) in poultry using conventional PCR. ILT is caused by an airborne viral pathogen caused by Gallid herpesvirus and is responsible for large economic losses. A major concern for the spread of ILT is the proximity of poultry farms to market gardens, where poultry manure is spread for fertiliser. The use of air sampling can provide a means of transmission tracking and monitoring.
The ability to air sample may provide an interesting additional sampling technique for the detection of airborne pathogens when coupled with Loop Mediated Isothermal Amplification (LAMP). LAMP is an isothermal method of amplifying nucleic acids that is becoming more routine for field use. The ability to air sample would allow the sentinel monitoring of things like spores and wind-borne pests. In addition to air borne pathogens, air sampling may prove useful for targets like spores and insects too. This research represents and exciting new avenue for the application of LAMP technology in the field.
GeneWorks and La Trobe University are part of a joint Co-operative Research Centre (CRCP) alongside WEHI, SI Systems and Bio2Lab to develop agriculture diagnostic kits using LAMP technology.
Learn more about the liquid cyclonic impinging device here.
Brown, L., Premaratna, D., Segal, Y. et al. Air sampling for detection of infectious laryngotracheitis (ILT) in commercial poultry flocks. BMC Res Notes 13, 556 (2020). https://doi.org/10.1186/s13104-020-05399-2