Opal 6-Plex Detection Kit (Whole Slide Imaging) - For Automatic Workflow

Formerly Known As: Opal Polaris 7 Color Automation IHC Detection Kit

The Opal 6-Plex Detection Kit for Whole Slide Imaging is part of the PhenoImager whole slide workflow.

This kit contains:
- 6 reactive fluorophores (Opal 480, Opal 520, Opal 570, Opal 620, Opal 690, and Opal 780)
- 10X Spectral DAPI
- DMSO
- 1X Plus Automation Amplification Diluent
- 1X Antibody Diluent/Block
- Opal Anti-Ms + Rb HRP

Users should source primary antibodies and other workflow reagents separately. The Opal 6-Plex Detection Kit - for Whole Slide Imaging was optimised for use on the Leica Biosystems BOND RX and configured and validated for imaging on the Vectra® Polaris™ quantitative pathology imaging system.

Kits contain enough reagents to stain up to 50-slides at the recommended 1:150 dilution ratio.

Opal will enable you to:
- Measure up to six tissue biomarkers at once
- Use the best primary antibodies together in multiplex panel, with no species-based crosstalk
- Retain spatial cellular context that is lost in flow cytometry
- Confirm single cell co-expression for many biomarkers in one tissue section
- Get more information while conserving precious tissue.

Automating Opal staining brings the benefit of:
- Quality, consistency and reproducibility with every sample
- Time-savings versus laborious manual process
- Ease-of-use with a walk-away, high-throughput protocol