Lymphocyte 3-plex Panel

Formerly Know as: Opal 4 Lymphocyte Kit

The Lymphocyte 3-Plex Panel enables phenotyping of helper T cells, cytotoxic T cells and B cells within intact FFPE tissue sections. Opal™ is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image, similar to standard immunohistochemistry (IHC).

The Lymphocyte 3-Plex Panel includes all critical workflow reagents to enable phenotyping of helper T cells, cytotoxic T cells and B cells within intact FFPE tissue sections with 3-plex fluorescent immunohistochemistry, including:

- Primary antibodies (CD4,CD8,CD20)
- Antibody diluent
- Fluorescent IHC detection reagents
- 10X Spectral DAPI
- Antigen retrieval buffers

This kit was validated for multispectral imaging on the Vectra and Mantra Systems and should also provide results with fluorescence microscopes that include emission filters for DAPI, FITC, CY3 and CY5. It contains enough reagents for a 3-plex immunostain of up to 50 slides (plus DAPI).

Optimised panel for assessment of immune cells in intact FFPE tissue, offering the shorterst path to results.

Part of the PhenoImager® workflow for immunophenotyping of solid tumors.