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FastGene Scriptase II cDNA 5x ReadyMix (100 rxns)

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Product Code: NGE-LS64

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The FastGene Scriptase II 5x ReadyMix is an engineered enzyme with a reduced RNase H activity and comes ready-to-use with all necessary ingredients to perform a reverse transcription. The FastGene Scriptase II has a modified RNase H domain. The RNA is therefore not degraded and serves as a template for longer cDNAs, resulting in fragment size of up to 12kBp.With its mutation in the RNase H domain and higher thermal stability, it is the optimal choice for more complex applications, such as RT-qPCR and NGS. The resulting full-length cDNA gives a complete picture of the gene and is able to show modification, e.g. splicing variants.

Applications:

- Quantification of Gene Expression

- RT-qPCR

- Next Generation Sequencing

- Low RNA concentration

- Difficult templates

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FastGene Scriptase II cDNA 5x ReadyMix (100 rxns)

Nippon Genetics

FastGene Scriptase II cDNA 5x ReadyMix (100 rxns)

Log in for pricing
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The FastGene Scriptase II 5x ReadyMix is an engineered enzyme with a reduced RNase H activity and comes ready-to-use with all necessary ingredients to perform a reverse transcription. The FastGene Scriptase II has a modified RNase H domain. The RNA is therefore not degraded and serves as a template for longer cDNAs, resulting in fragment size of up to 12kBp.With its mutation in the RNase H domain and higher thermal stability, it is the optimal choice for more complex applications, such as RT-qPCR and NGS. The resulting full-length cDNA gives a complete picture of the gene and is able to show modification, e.g. splicing variants.

Applications:

- Quantification of Gene Expression

- RT-qPCR

- Next Generation Sequencing

- Low RNA concentration

- Difficult templates

 

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