Thermostable Isothermal Master Mix (Dried - 300 rxns)

OptiGene’s novel isothermal mastermix for amplification reactions that require an initial heat-denaturing step.

• Suitable for HD-LAMP (Heat-denaturing LAMP).
• Contains the patented highly thermostable Tin(exo-) LF DNA Polymerase.
• Extreme thermostability and strong strand-displacement activity enable applications unachievable in a single, closed-tube reaction using alternative isothermal enzymes.
• Bulk economy packs avaliable (1500+ reactions)! E-mail customerservice@geneworks.com.au for quotation.

Key features:

• Extreme thermostability* ( < 98ºC )
• Strand displacement activity
• 65ºC temperature optimum
• 5’-3’ exonuclease minus
• 3’-5’ exonuclease minus

*no loss of activity is observed in a LAMP reaction following 5 minutes @ 95ºC step.

Advantages of HD-LAMP:

• Template denaturation step at 95ºC prior to amplification has been shown to increase target sensitivity¹
• Increased primer specificity – reduces false-priming that may occur at lower temperatures.
• Simple template extraction (cell-lysis).
• Sample heat-lysis, amplification and detection in a single tube.