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OptiGene
Phytophthora ramorum Assay Mastermix - 50rxns
Product Code:
OPG-PK-P.Ramorum-050W
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The Phytophthora ramorum Assay Mastermix (PK-P.Ramorum-050W) is designed to simplify the preparation of an isothermal amplification assay (LAMP). The master mix contains a proprietary fast, novel DNA polymerase, proprietary thermostable inorganic pyrophosphatase, optimised reaction buffer, MgSO4, dNTPs, ds-DNA binding dye (FAM detection channel) and separate optimised primers.
The GENIE detection system developed and manufactured by OptiGene allows real-time LAMP to be performed on a low-power portable platform, suitable for use in the field. The closed-tube nature of GENIE avoids any post-amplification handling thereby eliminating laboratory contamination with the amplified product.
This sudden oak death pathogen is known to infect over 130 plant species. Symptoms vary depending on the plant species affected. The disease cannot be identified based on field symptoms alone.
Phytophthora ramorum differs from most other Phytophthora species by affecting aerial parts of the plants rather than the roots.
Stability:
The Isothermal Mastermix wet reagents are stable for a minimum of twelve months when stored at -20ºC.
Supplied Reagents:
1 vial containing 50rxn isothermal mastermix
1 vial containing 50rxn of Phytophthora ramorum FOLIAR primer mix
Suggested Usage:
15µl Isothermal Master Mix
5µl Primer Mix
5µ Template
25µl Total Reaction Volume
Typical amplification conditions for this kit are:
Amplification 20mins 65ºC
Anneal 98ºC - 80ºC - ramping at 0.05ºC per second
Product anneal temperature; 87ºC +/- 1ºC
Positive Material Avaliable (OPG-CD-PRAM-050)
Further Reading:
Multiplex LAMP Detection of the Genus Phytophthora and Four Phytophthora Species P. ramorum, P. lateralis, P.kernoviae, and P. nicotainae, with a Plant Internal Control - (Hieno et al 2021)
The Phytophthora ramorum Assay Mastermix (PK-P.Ramorum-050W) is designed to simplify the preparation of an isothermal amplification assay (LAMP). The master mix contains a proprietary fast, novel DNA polymerase, proprietary thermostable inorganic pyrophosphatase, optimised reaction buffer, MgSO4, dNTPs, ds-DNA binding dye (FAM detection channel) and separate optimised primers.
The GENIE detection system developed and manufactured by OptiGene allows real-time LAMP to be performed on a low-power portable platform, suitable for use in the field. The closed-tube nature of GENIE avoids any post-amplification handling thereby eliminating laboratory contamination with the amplified product.
This sudden oak death pathogen is known to infect over 130 plant species. Symptoms vary depending on the plant species affected. The disease cannot be identified based on field symptoms alone.
Phytophthora ramorum differs from most other Phytophthora species by affecting aerial parts of the plants rather than the roots.
Stability:
The Isothermal Mastermix wet reagents are stable for a minimum of twelve months when stored at -20ºC.
Supplied Reagents:
1 vial containing 50rxn isothermal mastermix
1 vial containing 50rxn of Phytophthora ramorum FOLIAR primer mix
Suggested Usage:
15µl Isothermal Master Mix
5µl Primer Mix
5µ Template
25µl Total Reaction Volume
Typical amplification conditions for this kit are:
Amplification 20mins 65ºC
Anneal 98ºC - 80ºC - ramping at 0.05ºC per second
Product anneal temperature; 87ºC +/- 1ºC
Positive Material Avaliable (OPG-CD-PRAM-050)
Further Reading:
Multiplex LAMP Detection of the Genus Phytophthora and Four Phytophthora Species P. ramorum, P. lateralis, P.kernoviae, and P. nicotainae, with a Plant Internal Control - (Hieno et al 2021)
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