Bambanker DMSO Free (20mL)

• DMSO-free – for the most sensitive cells
• Enables long term cell storage at -80°C or -196°C
• Improved cell recovery and viability after thawing
• Ready-to-use freezing media – no programmed or sequential freezing required
• Serum-free – no risk of contamination
• GMP manufactured
• Usable for all know cell lines
• Long shelf life

No more DMSO – for the most sensitive cells

DMSO is added to most freezing reagents to help avoid formation of ice crystals, which of course harm the cells. However, DMSO is cytotoxic and can reduce the survival rate of certain sensitive cell lines. Bambanker DMSO Free is made without DMSO. Instead, it uses a unique formulation to avoid the formation of ice crystals. This makes Bambanker DMSO Free especially suitable for cell lines that proved to be sensitive to DMSO under long-term storage conditions.

Long-term storage of cultured cells

Cryopreservation of mammalian cells is extremely valuable and common in biological research. Fear of losing a cell line to contamination or incubator failure is frequently the impetus for making archival storage of cells a high priority after receiving or generating a new cell line. Once transferred from growth media to freezing medium, the cells are usually frozen at a controlled rate and stored in liquid nitrogen vapor or at -130°C in a mechanical deep freeze. Although freezing a cell line is a commonly performed procedure, problems arise when suitable freezers are not available, or unwanted variables are introduced by the presence of serum, extra-wash or complicated freezing algorithms.

Save time while saving your cells

The cell freezing media Bambanker™ permits cryopreservation of cells at -80°C (or in liquid nitrogen), obviating the need for an additional and expensive ultralow freezer and avoiding time consuming and complicated controlled freezing protocols. Simply (1) harvest cells, (2) aspirate medium, (3) resuspend in Bambanker™ (4) transfer to a cryovial and (5) store at -80°C. No programmed or sequential freezing is required! Bambanker™ is a serum-free cryopreservation medium that is delivered ready-to-use and can be kept in the refrigerator for up to two years. Convenient 20 ml bottles are available, making Bambanker™ freezing medium ideal for use by individual members of your lab. Today this innovative cell freezing medium Bambanker™ is the market leader in Japan and characterized by many different published articles with very sensitive cell lines all over the world.

Serum adds variation to long-term storage

All Bambanker™ products are produced with no serum. Cryopreservation media which contain sera have the disadvantage of fluctuations in recovery rates and undefined composition. Reproducibility of experiments with cells which were frozen in a serum-containing-medium, could be affected by lot-to-lot variation of the serum since the composition and concentration of proteins and other biological molecules varies with each batch of serum. This may result in issues when thawing and using cells from such serum-containing media. Breathe easy, as every ingredient of Bambanker™ is precisely defined so that you can be confident that cells stored at different times will all behave and recover similarly.

Flexible

There a four different version of Bambanker™:

- Bambanker (also avaliable in 20mL, 5x20mL, and 120mL sizes)

- Bambanker Direct (for hybridoma cells)

- Bambanker DMSO Free (for high sensitivity cells)

- Bambanker hRM (for ES and iPS cells)

GMP quality

All products of the Bambanker series are manufactured in facilities compliant with current good manufacturing practices (GMP).

The products are subject to a solid quality management system that ensures the consistency, reliability and high quality you can rely on. Documentation avaliable on request.