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Nippon Genetics
FastGene apTaq HotStart Polymerase (500 U)
Product Code:
NGE-LS34
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The FastGene apTaq DNA-Polymerase is a recombinant and thermostable Taq-Polymerase using the aptamer based Hot Start activation technology. The aptamer allows a reversible and immediate activation of the polymerase, leading to specific priming and a very fast PCR. The FastGene apTaq DNA-Polymerase is a Hot Start enzyme, which is completely inactive during room-temperature and become active only after heating. Random primer annealing and unspecific amplification that can occur with standard PCR enzymes are problems of yesterday. In contrast to antibody-based methods, the apTaq DNA-Polymerase contains synthetically manufactured aptamer-oligonucleotides. At low temperatures the polymerase is inactivated through a reversible tight bond of the aptamer. Interestingly, the aptamer acts as a molecular switch, changing its tertiary structure at higher temperatures. Temperatures below 45°C inactive the polymerase, whereas temperatures above 60°C fully activated the enzyme. Therefore, the FastGene apTaq DNA-Polymerase is less temperature-sensitive and reduce the risk of contamination.
The FastGene apTaq HotStart Polymerase is ideally suited for:
- Fast PCR
- Routine PCR
- PCR using complex templates
- SNP Analysis
- Any standard PCR application for which a hot start formulation of a high-quality thermostable DNA polymerase is required
The FastGene apTaq DNA-Polymerase is a recombinant and thermostable Taq-Polymerase using the aptamer based Hot Start activation technology. The aptamer allows a reversible and immediate activation of the polymerase, leading to specific priming and a very fast PCR. The FastGene apTaq DNA-Polymerase is a Hot Start enzyme, which is completely inactive during room-temperature and become active only after heating. Random primer annealing and unspecific amplification that can occur with standard PCR enzymes are problems of yesterday. In contrast to antibody-based methods, the apTaq DNA-Polymerase contains synthetically manufactured aptamer-oligonucleotides. At low temperatures the polymerase is inactivated through a reversible tight bond of the aptamer. Interestingly, the aptamer acts as a molecular switch, changing its tertiary structure at higher temperatures. Temperatures below 45°C inactive the polymerase, whereas temperatures above 60°C fully activated the enzyme. Therefore, the FastGene apTaq DNA-Polymerase is less temperature-sensitive and reduce the risk of contamination.
The FastGene apTaq HotStart Polymerase is ideally suited for:
- Fast PCR
- Routine PCR
- PCR using complex templates
- SNP Analysis
- Any standard PCR application for which a hot start formulation of a high-quality thermostable DNA polymerase is required
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