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Nippon Genetics
FastGene 2x IC Green Universal with Fluorescein (5000)
Product Code:
NGE-LS4150
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It is well-known that SYBR Green inhibits qPCR reactions. This fact led to the development of SYBR resistant enzymes. An alternative approach is to develop a dye that does not inhibit the reaction. This dye is named FastGene IC Green. This is an intercalating dye, only detecting double stranded DNA. By not inhibiting the reaction, the FastGene IC Green kit is able to detect genes at a lower CT-value, creating a higher sensitivity! The superior buffer chemistry enables the detection of low copy number genes, which with other dyes could not be detected.
The FastGene IC Green buffers were designed to have a superior robustness. This guarantees the linearity of the qPCR reaction, thus improving accuracy which is essential for reproducible results. Additionally, qPCRs can be performed at shorter amplifcation times i.e. using fast protocols. The FastGene IC Green kit is universal. The reference dyes come in a separate vial and can be added once to the master mixes. Hence, this kit can be used with qPCR instruments which need a high ROX concentration as well as instruments that need a low concentration or no ROX. There is even a special version with fluorescein.
Applications:
-Quantification of Gene Expression
-Quantification of Gene Copy number
-Melt-curve analysis
-Detection of Gene Expression (Knock-out analysis)
It is well-known that SYBR Green inhibits qPCR reactions. This fact led to the development of SYBR resistant enzymes. An alternative approach is to develop a dye that does not inhibit the reaction. This dye is named FastGene IC Green. This is an intercalating dye, only detecting double stranded DNA. By not inhibiting the reaction, the FastGene IC Green kit is able to detect genes at a lower CT-value, creating a higher sensitivity! The superior buffer chemistry enables the detection of low copy number genes, which with other dyes could not be detected.
The FastGene IC Green buffers were designed to have a superior robustness. This guarantees the linearity of the qPCR reaction, thus improving accuracy which is essential for reproducible results. Additionally, qPCRs can be performed at shorter amplifcation times i.e. using fast protocols. The FastGene IC Green kit is universal. The reference dyes come in a separate vial and can be added once to the master mixes. Hence, this kit can be used with qPCR instruments which need a high ROX concentration as well as instruments that need a low concentration or no ROX. There is even a special version with fluorescein.
Applications:
-Quantification of Gene Expression
-Quantification of Gene Copy number
-Melt-curve analysis
-Detection of Gene Expression (Knock-out analysis)
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