FastGene IC Green One Step Mix Hi-ROX (100)

The FastGene IC Green 1-Step mix contains a reverse transcriptase and a DNA polymerase. Having a 1-tube reaction setup for the reverse transcription and for the quantitative PCR has many advantages:

- The 2x master mix ensures the same concentration of buffer and enzyme in every reaction

- More convenient and saves time

The FastGene IC Green 1-Step mix contains the same non-inhibiting dye as the original FastGene IC Green mixes. Hence, it can detect low-copy genes. During the first part of the reaction, RNA is reverse transcribed with a powerful reverse transcriptase enzyme. Afterwards, a DNA Taq polymerase amplifies the DNA using gene specific primers. The superior buffer design ensures the highest processivity of each enzyme. The FastGene IC Green 1-Step mix is therefore compatible with fast and ultra-fast cycling conditions.

Applications:

- Melt-curve analysis

- Quantification of Gene Expression

- Quantification of Gene Copy number

- Detection of Gene Expression (Knock-out analysis)