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Nippon Genetics
FastGene Scriptase II (20000 U)
Product Code:
NGE-LS53
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The FastGene Scriptase II is an engineered enzyme with a reduced RNase H activity and comes ready-to-use with all necessary ingredients to perform a reverse transcription. The FastGene Scriptase II has a modified RNase H domain. The RNA is therefore not degraded and serves as a template for longer cDNAs, resulting in fragment size of up to 12kBp.With its mutation in the RNase H domain and higher thermal stability, it is the optimal choice for more complex applications, such as RT-qPCR and NGS. The resulting full-length cDNA gives a complete picture of the gene and is able to show modification, e.g. splicing variants.
Applications:
- Quantification of Gene Expression
- RT-qPCR
- Next Generation Sequencing
- Low RNA concentration
- Difficult templates
The FastGene Scriptase II is an engineered enzyme with a reduced RNase H activity and comes ready-to-use with all necessary ingredients to perform a reverse transcription. The FastGene Scriptase II has a modified RNase H domain. The RNA is therefore not degraded and serves as a template for longer cDNAs, resulting in fragment size of up to 12kBp.With its mutation in the RNase H domain and higher thermal stability, it is the optimal choice for more complex applications, such as RT-qPCR and NGS. The resulting full-length cDNA gives a complete picture of the gene and is able to show modification, e.g. splicing variants.
Applications:
- Quantification of Gene Expression
- RT-qPCR
- Next Generation Sequencing
- Low RNA concentration
- Difficult templates
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