GWS-K-BACT-08-10

Brettanomyces bruxellensis LAMP Detection Kit

Brettanomyces bruxellensis is a spoilage yeast responsible for significant economic losses in the global and Australian wine industries. The organism produces volatile phenolic compounds, principally 4-ethylphenol and 4-ethylguaiacol, imparting undesirable 'Brett' characters described as barnyard, medicinal, or leather-like aromas that render wine commercially unacceptable. It thrives in barrel-aged red wines and is prevalent across Australian wine regions. The yeast is remarkably resistant to sulfur dioxide and extreme pH, making cellar management challenging. Early and accurate detection is essential to implement corrective winemaking practices and protect Australia's economically valuable wine industry.

Loop-Mediated Isothermal amplification (LAMP) is a molecular biology technique that copies DNA or RNA at a single, constant temperature. By eliminating the rapid heat cycling required by traditional PCR, this method relies on specialized strand-displacement polymerases to unzip and replicate genetic material.

This biological approach removes the need for expensive, bulky thermal cyclers, allowing assays to run on simple, low-cost heating equipment. Delivering highly sensitive results in just 20 to 30 minutes, isothermal amplification provides a fast, affordable, and exceptionally portable solution for field testing and decentralized point-of-care diagnostics.

The Brettanomyces bruxellensis LAMP Detection Kit simplifies detection of Brettanomyces bruxellensis nucleic acid by amplifying target DNA at a constant temperature. 

• All-in-one box: reagents and plasticware included
  
  
• Results in under 30 min
  
  
• Developed in collaboration with AWRI (Australian Wine Research Institute and Wine Australia

Platform: Genie LITE, Genie III and Genie HT

Application: Nucleic Acid detection of Brettanomyces bruxellensis

Target Analyte: bacterial DNA

Technology: LAMP